Tylophora hirsuta (Wall.) extracts ameliorates diabetes associated with inflammation in alloxan induced diabetic rats

Razzaque, Faisal and Sharif, Ali and Akhtar, Bushra and Khan, Humaira Majeed and Akhtar, Muhammad Furqan and Zaib, Maryam and Muhammad, Abdullah and Sohail, Kashif and Hamid, Irfan and Qaisar, Naeem (2020) Tylophora hirsuta (Wall.) extracts ameliorates diabetes associated with inflammation in alloxan induced diabetic rats. Endocrine, Metabolic & Immune Disorders - Drug Targets.

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Abstract

BACKGROUND:
Tylophora hirsuta Wall. has long been used as traditional medicine for the treatment of diabetes. Current study is designed to evaluate the antidiabetic and anti-inflammatory activity of different extracts of aerial parts of Tylophora hirsuta.
METHODS:
Sequential maceration was conducted to obtain extracts. Total phenolic contents were determined by FolinCiocalteau method. The antioxidant activity was assessed by DPPH free radical scavenging assay. The extracts were tested for its inhibitory activity against α-amylase in-vitro. In-vivo anti diabetic assay was conducted using alloxan induced diabetic model and OGTT was conducted on normal rats. ELISA was used to determine the proinflammatory cytokines (TNF-α and IL-6). Polyphenolic composition of the extract was analyzed using a HPLC system.
RESULTS:
Aqueous extract exhibited highest total phenolic contents (985.24±3.82 mg GAE/100 g DW), antioxidant activity (IC50= 786.70±5.23.

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