Chemical characterisation, in vitro antioxidant, cytotoxicity and safety evaluation of Polystichum braunii (Spenn.) fee roots

Saleem, Ammara and Saleem, Mohammad and Akhtar, Muhammad Furqan and Rasul, Azhar and Baig, Mirza Muhammad Faran Ashraf (2020) Chemical characterisation, in vitro antioxidant, cytotoxicity and safety evaluation of Polystichum braunii (Spenn.) fee roots. NATURAL PRODUCT RESEARCH.

Text (Research Article) 08 Chemical characterisation in vitro antioxidant cytotoxicity and safety evaluation of Polystichum braunii Spenn fee roots.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

The study was intended to find in vitro antioxidant, cytotoxicity and safety profile of Polystichum braunii roots extracts and chemically characterise it by GC–MS and FTIR spectroscopy. Acute and sub-chronic (90-day oral repeated dose at 150, 300 and 600 mg/kg) toxicity studies of P. braunii methanol extract (PBME) were performed in Wistar rats by adopting OECD guidelines. The PBME exerted the highest in vitro antioxidant potential. There was no substantial change in blood parameters and body weight except a notable (p<0.01) rise in ALT, ALP, MDA and HDL, and decrease in cholesterol and LDL (p<0.05) in animals sub-chronically treated with PBME at 600mg/kg dose. The IC50 in HepG2 was more than 1000 mg/ml. The GC–MS analysis revealed Pentadecanoic acid and 9,12-Octadecadienoic acid in PBME. The P. braunii might be safe in acute usage, however, monitoring of liver function tests should be carried out during long-term use.

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