Reverse Phase High Performance Liquid Chromatographic-Diode Array Detection Method for Quantitation of Rhein in Micro sample of Rabbit Plasma and Application to the Pharmacokinetic Study

Madni, Asad Ullah and Imran Khan, Muhammad and Rehman, Mubashar and Tahir, Nayab Reverse Phase High Performance Liquid Chromatographic-Diode Array Detection Method for Quantitation of Rhein in Micro sample of Rabbit Plasma and Application to the Pharmacokinetic Study. Reverse Phase High Performance Liquid Chromatographic-Diode Array Detection Method for Quantitation of Rhein in Micro sample of Rabbit Plasma and Application to the Pharmacokinetic Study, 42 (6). pp. 866-874. ISSN 0253-5106

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Abstract

Summary: The present work describes a reversed phase high-performance liquid chromatography (RP-HPLC) assay with diode array detection (DAD) for the determination of rhein, an active metabolite of diacerein, in spiked rabbit plasma. The plasma samples were subjected to one-step liquid-liquid extraction using a protein precipitating agent. The separation of rhein was achieved on an RP C18 hypersil-ODS column (250×4.6mm, 5μm). The mobile phase consisted of a binary mixture of methanol and water (15: 85 v/v, pH 4.7) run under the isocratic system at a flow rate of 1 ml.min-1, the wavelength of DAD was set at 210 nm. The method was validated for linearity, accuracy, precision, and stability according to the International Council for Harmonization (ICH guidelines). The calibration curve was linear over the range of 0.015-5 μg. ml-1 for rhein with the coefficient of regression (r2) of value 0.9980. Extraction recovery for rhein from rabbit plasma was in the range of 95.0% to 98.60% to different sample concentrations. The retention time of rhein was observed at 5.34 min. Limit of detection (LOD) and limit of quantification (LOQ) were found to be 5 ng.ml-1 and 15 ng.ml-1 respectively. The assay method was successfully applied to the pharmacokinetic study of diacerein plain aqueous dispersion in albino rabbits.

Item Type:	Article
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Pharmaceutical Sciences (FPS) > Riphah Institute of Pharmaceutical Sciences Lahore
Depositing User:	Dr Muhammad Imran Khan
Date Deposited:	18 Feb 2021 14:07
Last Modified:	18 Feb 2021 14:07
URI:	http://research.riphah.edu.pk/id/eprint/1186

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