A COMPARATIVE CYTOMORPHOMETRIC STUDY OF BUCCAL MUCOSAL SMEARS OF CIGARETTE SMOKERS AND NASWAR (NICOTIANA TABACUM) USERS

MASOOD, R. and JAFFAR, R. and ZAIB, N. and UMER, N. and JAVED, R.M. (2017) A COMPARATIVE CYTOMORPHOMETRIC STUDY OF BUCCAL MUCOSAL SMEARS OF CIGARETTE SMOKERS AND NASWAR (NICOTIANA TABACUM) USERS. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 124 (3). e203. ISSN 22124403

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Abstract

Objective: To determine and compare cytomorphometric
changes in buccal mucosal cells of cigarette smokers, naswar
users and non-users/smokers.
Materials and Methods: Cellular diameter CD, nuclear
diameter ND and nuclear to cytoplasmic ratio N/C ratio were
assessed in buccal smears taken with wooden spatula from clinically normal mucosa of smokers, naswar users and control group.
The sample size was 99 subjects of ages 15 years-60years,
divided into three groups (33 each group) as M, S and N i.e.
control, smokers and naswar users respectively. Slides were
stained with three stains Hematoxylin and Eosin Stain, Giemsa
Stain and Papanicolaou Stain.
Results: The cytomorphometric variables were measured
by using stage and ocular micrometers. The mean cellular
diameter of group M, S and N was 43.8mm, 54.3mm and 42.7mm
respectively. The mean nuclear diameter of M, S and N was
9.97mm, 12.6mm and 11.8mm respectively. And the mean N/C
ratio of group M, S and N was 1:4.4, 1:4.3and 1:3.5 respectively.
The mean differences between CD, ND and N/C ratio in all three
stains, among the three groups, S, N and M was found to be
statistically highly significant i.e. p ¼ 0.001 on ONE WAY
ANOVA. While, on Post hoc tukey test between S and N, CD and
N/C ratio were highly significant p¼0.000 while ND was not
significant. While between S and M, CD and ND both were
significant and showed p¼0.000 while N/C ratio was not significant. In comparison between N and M, ND and N/C ratio were
found to be significant while CD was not significant.
Conclusion: The cytomorphometric changes assessed by
this study depict only cause effect relationship with smoking and
naswar use. Association of these changes with dysplasia or premalignancy needs further verification with the help of specific
immune-markers.

Item Type:	Article
Subjects:	R Medicine > RK Dentistry
Divisions:	Faculty of Health and Medical Sciences (FHMS) > Islamic International Dental College Islamabad
Depositing User:	Dr Kanwal Sohail
Date Deposited:	10 Mar 2020 06:17
Last Modified:	10 Mar 2020 06:17
URI:	http://research.riphah.edu.pk/id/eprint/282

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