Waseem, Muhammad and khan, Irfan and Iqbal, Hana'a and Eijaz, Sana and Usman, Shumaila and Ahmed, Nazia and Alam, Gulzar and Salim, Asmat (2016) Hypoxic Preconditioning Improves the Therapeutic Potential of Aging Bone Marrow Mesenchymal Stem Cells in Streptozotocin-Induced Type-1 Diabetic Mice. Cellular Reprgramming, 18 (5). pp. 344-355. ISSN 2152-4998
Full text not available from this repository. (Request a copy)Abstract
Insulin replacement is the current therapeutic option for type-1 diabetes. However, exogenous insulin cannot
precisely represent the normal pattern of insulin secretion. Another therapeutic strategy is transplantation of
pancreatic islets, but this is limited by immune rejection, intrinsic complications, and lack of donor availability.
Stem cell therapy that results in the regeneration of insulin-producing cells represents an attractive choice.
However, with advancing age, stem cells also undergo senescence, which leads to changes in the function of
various cellular processes that result in a decrease in the regeneration potential of these aging stem cells. In this
study, the effect of young and aging mesenchymal stem cells (MSCs) on the regeneration of pancreatic beta
cells in streptozotocin (STZ)-induced type-1 diabetic mice was observed after hypoxic preconditioning. Hypoxia
was chemically induced by 2, 4-dinitrophenol (DNP). Plasma insulin and glucose levels were measured at
various time intervals, and pancreatic sections were analyzed histochemically. The effect of DNP was also
analyzed on apoptosis of MSCs by flow cytometry and on gene expression of certain growth factors by
quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). We observed that hypoxic
preconditioning caused changes in the gene expression levels of growth factors in both young and aging MSCs.
Young MSCs showed significant regeneration potential compared with the aging cells in vivo. However,
hypoxic preconditioning was able to improve the regeneration potential of aging MSCs. It is concluded from the
present study that the regeneration potential of aging MSCs into pancreatic b-cells can be enhanced by hypoxic
preconditioning, which causes changes in the gene expression of certain growth factors.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QP Physiology |
| Divisions: | Faculty of Rehabilitation and Allied Health Sciences(FRAHS) > Riphah College of Rehabiliation and Allied Sciences Islamabad |
| Depositing User: | Dr Muhammad Waseem |
| Date Deposited: | 17 Jul 2020 06:44 |
| Last Modified: | 17 Jul 2020 06:44 |
| URI: | http://research.riphah.edu.pk/id/eprint/582 |
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